研究了龙山荞、浦江荞、美国荞和牡丹荞四种甜荞子叶和下胚轴的愈伤组织形成与分化的影响因子.结果表明:17.8μmol/L~26.6μmol/L(4.0 mg/L~6.0 mg/L)的BA与0.9μmol/L(0.2 mg/L)的2,4-D配合使用可有效促进甜荞愈伤组织中芽的分化和芽的生长;高浓度的蔗糖(4.5%~6.0%)促进甜荞愈伤组织根的分化和愈伤组织的生长;在培养基中附加活性炭抑制甜荞愈伤组织分化根. The effects of 17.8μmol / L ~ 26.6μmol / L (4.0 mg / L) on the callus formation and differentiation of four kinds of buckwheat leaves and hypocotyls of Longshan buckwheat, Pujiang buckwheat, ~ 6.0 mg / L BA and 0.9 μmol / L 2,4-D (0.2 mg / L) could effectively promote bud differentiation and shoot growth in callus of Tartary buckwheat. High concentrations of sucrose (4.5 % ~ 6.0%) promoted callus differentiation and callus growth of callus; addition of activated carbon in culture medium inhibited callus differentiation of callus.