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为探讨海马放射状胶质细胞在切割穹窿海马伞侧海马提取液的诱导下形态发生的变化,将培养的海马胶质细胞接种于24孔培养板中,分成诱导组和对照组,诱导组加入含5%切割穹窿海马伞侧海马提取液的DMEM/F12培养液,对照组加入单纯的细胞培养液,分别于培养后1、3、7和14d时行BLBP免疫荧光检测和Hoechst标记。计算两组各天时BLBP阳性细胞占Ho-echst阳性细胞的百分比,并用LeicaQiwn图像处理软件检测BLBP阳性细胞的周长和面积(含突起)。Stata8.0统计软件行组间比较。结果显示,1d时诱导组BLBP阳性细胞的比例稍高于对照组,两组细胞胞体均较小,突起均较短较细,无明显差异;3d时,诱导组BLBP阳性细胞的比例明显高于对照组,且胞体较大,突起较粗较长;7d时诱导组BLBP阳性细胞的比例明显高于对照组达到高峰,胞体更大,突起更粗更长交织成网;14d时两组BLBP阳性细胞的比例均稍有降低,但诱导组的比例仍明显高于对照组,两组细胞的胞体稍变小,突起稍变细变短。上述结果提示,切割穹窿海马伞侧海马提取液可明显地诱导BLBP阳性放射状胶质细胞增殖,并使胞体变大,突起变粗变长,呈“激活”状态。
In order to investigate the morphological changes of hippocampal radial glial cells induced by cutting the hippocampus of the hippocampus of the hippocampus, the cultured hippocampus glial cells were inoculated into 24-well culture plates and divided into induction group and control group. The DMEM / F12 culture medium of the hippocampus of the hippocampus of 5% was cut, and the control group was added with simple cell culture medium. BLBP immunofluorescence and Hoechst labeling were performed at 1, 3, 7 and 14 days after culture respectively. The percentage of BL-B positive cells to Ho-echst-positive cells in each group was calculated. The peripheral length and area (including protrusions) of BLBP-positive cells were detected by LeicaQiwn image processing software. Stata8.0 statistical software to compare between groups. The results showed that the proportion of BLBP-positive cells in the induced group was slightly higher than that in the control group at 1 day. The cell bodies of both groups were smaller and the protrusions were shorter and smaller. There was no significant difference between the two groups. At 3d, the percentage of BLBP positive cells in induction group was significantly higher The control group had a large cell body and thicker protuberances. On the 7th day, the percentage of BLBP-positive cells in the induced group was significantly higher than that in the control group, the cell bodies were larger and the protuberances were thicker and longer. The proportion of cells were slightly lower, but the proportion of induction group was still significantly higher than the control group, the two groups of cells slightly smaller cell body, protrusion slightly thinner and shorter. These results suggest that the hippocampus extract from the fimbria of the hippocampus can significantly induce the proliferation of BLBP-positive astrocytes and enlarge the cytoplasm. The protuberances become thicker and longer and become “activated”.