为有效提取香菇中的可溶性含氮化合物,通过对高速组织捣碎机、胶体磨和高压均质机3种机械物理破壁的比较,最终确定采用高压均质机以达到最佳的破壁效果,从而释放菌体细胞内大量的可溶性含氮化合物。通过对均质工艺单因素及正交实验的探讨,得到最适工艺条件为均质压力40 MPa、均质温度50℃、均质料液比1∶7、均质次数2次,可溶性氮释放率可达到78.84%。 In order to effectively extract soluble nitrogen compounds in mushrooms, through the comparison of three kinds of mechanical and physical breaking of high-speed tissue stamping machine, colloid mill and high-pressure homogenizer, the best breaking effect is achieved by using high-pressure homogenizer , Thereby releasing a large number of soluble nitrogen compounds in bacterial cells. Through the discussion of single factor and orthogonal experiment of homogenization process, the optimum process conditions were as follows: homogenization pressure 40 MPa, homogenization temperature 50 ℃, homogeneous material-liquid ratio 1: 7, homogenization times 2, soluble nitrogen release rate Can reach 78.84%.