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从大鼠的尾壳核组织中提取总 RNA,以 RT-PCR方法扩增出大鼠 m Glu R5 长度约 43 5 bp的 c DNA片段。将这一片段克隆到 PGEM-T载体中进行序列分析 ,结果证实所克隆的 c DNA是编码正确的大鼠 m Glu R5 的一段基因序列。克隆的这段大鼠m Glu R5 特异性基因片段可用于制作探针 ,利用原位杂交技术检测其 m RNA在正常或异常状况下的表达 ;也可制作反意 c DNA或反意 m RNA以研究 m Glu R5 在生理或病理状态下的作用 ;还可进行反意 c DNA或反意 m RNA基因治疗。总而言之 ,克隆的这段基因 ,对研究 m Glu R5 在生理及病理条件下的功能变化 ,以及对与其有关疾病的基础研究和临床应用具有重要意义
Total RNA was extracted from the caudate putamen nucleus of rats and a c DNA fragment of about 43 5 bp in length was obtained by RT-PCR. This fragment was cloned into the PGEM-T vector for sequence analysis. As a result, it was confirmed that the cloned cDNA was a gene sequence encoding the correct rat mGluR5. Cloning of this rat mGluR5 specific gene fragments can be used to make probes, in situ hybridization detection of m RNA under normal or abnormal expression; also can be made of anti-c DNA or anti-m RNA To study the role of mGluR5 in physiological or pathological conditions and to gene therapy of anti-c DNA or anti-m RNA. In summary, this cloned gene is important for studying the functional changes of mGluR5 in physiological and pathological conditions, as well as in the basic research and clinical application of the disease associated with it