Background:Tree mortality and regeneration(seedling and sapling recruitment)are essential components of forest dynamics in arid regions,especially where subjected to serious eco-hydrological problems.In recent decades,the mortality of the Euphrates poplar(Populus euphratica)along the Tarim River in Northwest China has increased.However,few studies have quantified the causes of mortality and regeneration in this azonal riparian forest type.Methods:The present study describes the annual hydrological response of tree mortality and regeneration in forest gaps.A total of 60 canopy gaps were investigated in six replicate grid plots(50 m x 50 m)and the annual runoff and water consumption data during the period of 1955-2016 were collected from hydrological stations in the middle reaches of the Tarim River.We compared the regeneration density of seedlings and saplings within the canopy gap areas(CGAs),undercanopy areas(UCAs),and uncovered riverbank areas(RBAs)through detailed field investigation.Results:Our study found that the mortality of young and middle-aged gap makers has increased remarkably over recent decades,particularly since the year 1996.The main results indicated that regional water scarcity was the primary limiting factor for long-term changes in tree mortality,as shown by a significant correlation between the diameter at breast height(DBH)of dead trees and the annual surface water.The average density(or regeneration rate)of seedlings and saplings was highest in the RBAs,intermediate in the CGAs,and lowest in the UCAs.Compared with the UCAs,the CGAs promote tree regeneration to some extent by providing favorable conditions for the survival and growth of seedlings and saplings,which would otherwise be suppressed in the understory.Furthermore,although the density of seedlings and saplings in the CGAs was not as high as in the RBAs,the survival rate was higher in the CGAs than in the RBAs.Conclusion:Forest canopy gaps in floodplain areas can play a decisive role in the long-term germination and regeneration of plant species.However,as a typical phreatophyte in this hyper-arid region,the ecosystem structure,functions and services of this fragile P.euphratica floodplain forests are threatened by a continuous decrease of water resources,due to excessive water use for agricultural irrigation,which has resulted in a severe reduction of intact poplar forests.Furthermore,the survival of seedlings and saplings is influenced by light availability and soil water at the regional scale.Our findings suggest that policymakers may need to reconsider the restoration and regeneration measures implemented in riparian P.euphratica forests to improve flood water efficiency and create canopy gaps.Our results provide with valuable reference information for the conservation and sustainable development of floodplain forest ecosystems.