目的　探讨雷公藤T4单体和干扰素α -2b对人成纤维细胞增殖作用的影响。方法　人成纤维细胞常规传代培养。用比色法检测分别加入雷公藤T4单体、干扰素α -2b、 5-氟尿嘧啶及T4加 5-氟尿嘧啶后对细胞增殖的影响。结果　雷公藤T4单体、 5-氟尿嘧啶及T4加5-氟尿嘧啶对人成纤维细胞增殖有抑制作用。干扰素α -2b对人成纤维细胞增殖有促进作用。结论　雷公藤T4单体对实验性人成纤维细胞的增殖有抑制作用 Objective To investigate the effects of tripterygium wilfordii T4 monomer and interferon α -2b on the proliferation of human fibroblasts. Methods Human fibroblasts were subcultured routinely. The colorimetric method was used to detect the effect of Tripterygium T4 monomer, interferon α-2b, 5-fluorouracil and T4 plus 5-fluorouracil on cell proliferation. Results Tripterygium T4 monomer, 5-fluorouracil and T4 plus 5-fluorouracil inhibited the proliferation of human fibroblasts. Interferon α -2b can promote the proliferation of human fibroblasts. Conclusion Triptolide T4 can inhibit the proliferation of human fibroblasts