载脂蛋白CⅡ(apo CⅡ)主要存在于乳糜微粒及VLDL之中,是脂蛋白脂酶不可缺少的激活剂,在富含甘油三酯的脂蛋白(乳糜微粒及VLDL)的代谢中具有特别重要的作用。作者采用超速离心和SephadexG-75以及DEAE-Sephacel层析由人血浆制备纯化的apo CⅡ,建立了一种特异、灵敏、简便的人血清apo CⅡ竞争性酶免疫测定法(Competitive En-zyme Immunoassay,CEIA)。单价特异抗体由免 Apo C II is found in chylomicrons and VLDL and is an indispensable activator of lipoprotein lipase. It is particularly important in the metabolism of triglyceride-rich lipoproteins (chylomicrons and VLDL) Role. The authors prepared purified apo CⅡ from human plasma by ultracentrifugation, Sephadex G-75 and DEAE-Sepharose chromatography and established a specific, sensitive and simple competitive ELISA method for competitive human serum apo CⅡ (Competitive En-zyme Immunoassay, CEIA). Monovalent specific antibodies by free