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目的:建立测定苦参碱微球包封率的HPLC法。方法:使用Fortis Xi C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇-乙腈-3%磷酸溶液(11∶80∶9)为流动相,流速:0.6 ml·min-1,检测波长:210 nm,柱温:25℃,进样量:20μl。结果:苦参碱在3~150μg·ml-1浓度范围内峰面积与浓度呈良好的线性关系(r=0.999 7),回收率为99.92%(RSD=0.97%,n=9),制备的苦参碱微球包封率为(81.85±3.22)%(n=3)。结论:该方法可用于测定苦参碱微球的包封率。
Objective: To establish an HPLC method for determination of encapsulation efficiency of matrine microspheres. METHODS: Fortis Xi C18 (250 mm × 4.6 mm, 5 μm) column was used with a mobile phase of methanol-acetonitrile-3% phosphoric acid solution (11:80:9) at a flow rate of 0.6 ml · min- 210 nm, column temperature: 25 ℃, injection volume: 20μl. Results: The peak area of matrine in the concentration range of 3 ~ 150μg · ml-1 was linear with the concentration (r = 0.999 7) and the recovery was 99.92% (RSD = 0.97%, n = 9) Matrine microsphere encapsulation efficiency was (81.85 ± 3.22)% (n = 3). Conclusion: This method can be used to determine the entrapment efficiency of matrine microspheres.