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目的 观察端粒酶逆转录酶 (hTERT)基因反义寡核苷酸 (ASODN)对肺癌细胞端粒酶活性和细胞凋亡的影响。方法 实验分为ASODN组、正义寡核苷酸 (SODN)组和空白对照组 ,所用ASODN和SODN的浓度分别为 10 μmol/L ,脂质体为 16mg/L ,采用端粒重复扩增法、逆转录 聚合酶链反应、WesternBlot及流式细胞术分别观察各组端粒酶活性、hTERTmRNA和蛋白质表达以及细胞凋亡。结果 ASODN组显著下调或抑制肺癌细胞端粒酶活性和hTERT表达 ,但直到第 2 1天才出现细胞凋亡增多。结论 端粒酶活性与hTERT表达密切相关
Objective To observe the effect of telomerase reverse transcriptase (hTERT) gene antisense oligonucleotide (ASODN) on telomerase activity and apoptosis in lung cancer cells. Methods The experiment was divided into two groups: ASODN group, SOD group and blank control group. The concentrations of ASODN and SODN were 10 μmol / L and 16 mg / L, respectively. The telomere repeat amplification Reverse transcriptase polymerase chain reaction, Western blot and flow cytometry were used to observe the telomerase activity, hTERT mRNA and protein expression and apoptosis respectively. Results The ASODN group significantly down-regulated or inhibited the telomerase activity and hTERT expression in lung cancer cells. However, apoptosis increased only on the 21st day. Conclusion Telomerase activity is closely related to hTERT expression