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目的:通过检测瞬时受体阳离子通道亚家族V成员6(TRPV6)和Ki-67在胃腺癌、胃腺瘤和正常胃组织中的表达,了解TRPV6的表达与胃腺癌临床病理特征的关系,探讨其在胃腺癌发生发展中的作用。方法:收集手术切除的胃腺癌组织标本68例、胃腺瘤组织标本30例、正常胃组织标本20例,应用免疫组化Eli VisionTMplus法检测TRPV6和Ki-67在不同组织中的表达。结果:TRPV6在正常胃组织、胃腺瘤和胃腺癌中阳性表达率逐渐升高,在胃腺癌与正常胃组织、胃腺瘤与正常胃组织中表达差异均有统计学意义(P<0.05),在胃腺癌与胃腺瘤组织中表达差异无统计学意义(P>0.05);Ki-67抗原在正常胃组织、胃腺瘤和胃腺癌中阳性表达逐渐升高(P<0.05);TRPV6在胃腺癌中的表达与肿瘤分期、有无淋巴结转移及Ki-67的阳性指数相关(P<0.05)。结论:TRPV6在胃腺癌和胃腺瘤中的表达升高,提示其在胃腺癌的发生发展中发挥作用,可作为胃癌重要的生物学标志,联合Ki-67抗原检测对胃癌的诊断、预后评估具有重要意义。
OBJECTIVE: To investigate the relationship between the expression of TRPV6 and the clinicopathological features of gastric adenocarcinoma by detecting the expression of transient receptor cation channel subfamily V-member 6 (TRPV6) and Ki-67 in gastric adenocarcinoma, gastric adenoma and normal gastric tissues In the occurrence and development of gastric adenocarcinoma. Methods: 68 cases of gastric adenocarcinoma, 30 cases of gastric adenoma and 20 cases of normal gastric tissue were collected. The expression of TRPV6 and Ki-67 in different tissues were detected by immunohistochemistry Eli VisionTMplus method. Results: The positive rate of TRPV6 in normal gastric tissue, gastric adenoma and gastric adenocarcinoma increased gradually, and there was significant difference between gastric adenocarcinoma and normal gastric tissue, gastric adenoma and normal gastric tissue (P <0.05) The positive expression of Ki-67 in normal gastric tissue, gastric adenoma and gastric adenocarcinoma was significantly increased (P <0.05). The expression of TRPV6 in gastric adenocarcinoma was significantly higher than that in gastric adenocarcinoma (P0.05) The expression of Ki-67 was correlated with tumor stage, lymph node metastasis and Ki-67 positive index (P <0.05). Conclusion: The increased expression of TRPV6 in gastric adenocarcinoma and gastric adenocarcinoma suggests that TRPV6 plays a role in the development of gastric adenocarcinoma and may be used as an important biomarker of gastric cancer. The detection of TRPV6 in gastric adenocarcinoma and gastric adenoma with prognosis Significance.