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目的 了解巨细胞病毒肝炎 (CMV IgM抗体阳性 )和丙型肝炎 (HCV IgM抗体阳性 ) 1～ 6月的婴儿患者血清中某些炎性介质的异常变化。方法 应用ELISA法和硝酸还原酶法 ,分别检测了 5 6例CMV抗体和6 7例HCV抗体阳性婴儿肝炎综合征 (婴肝 )患儿及正常对照组 5 8例小儿的血清干扰素α (IFN α) ,白细胞介素 8(IL 8) ,肿瘤坏死因子 α (TNF α)和一氧化氮 (NO)的含量。结果 正常对照组 5 8例血清IFN α ,IL 8,TNF α ,NO含量分别为 2 2 6 .74± 73.82ng/L ,13.2 4± 5 .36ng/L ,2 17.14± 76 .30ng/L ,2 5 .98± 8.70 μmol/L。CMV抗体阳性婴肝组血清IFN α ,IL 8,TNF α和NO的含量分别为 5 82 .2 6± 131.72ng/L ,75 .2 8± 33.5 7ng/L ,42 9.46± 15 6 .32ng/L和5 9.87± 16 .42 μmol/L ,明显高于正常对照组 (P <0 .0 1) ;HCV抗体阳性婴肝组上述四项指标含量分别为5 5 8.32± 114.6 4ng/L ,71.34± 2 7.6 4ng/L ,374.35± 138.4ng/L和 6 2 .2 4± 2 1.38μmol/L ,其含量亦明显高于正常对照组 (P <0 .0 0 1)。CMV与HCV抗体阳性婴肝组比较 ,无明显差异 (P >0 .0 5 )。TNF α和NO在这两组婴肝中分别呈正相关 (r1=0 .6 2 ,r2 =0 .5 7,P<0 .0 1)。结论 CMV和HCV抗体阳性婴肝急性期患儿 ,血清TNF α,IL 8,IFN
Objective To understand the abnormal changes of some inflammatory mediators in sera of patients with cytomegalovirus hepatitis (CMV IgM antibody positive) and hepatitis C virus (HCV IgM antibody positive) from January to June. Methods ELISA and nitrate reductase were used to detect the serum level of interferon alpha (IFN-α) in 56 infants with hepatitis C syndrome (infant liver) and 56 normal controls α, IL 8, TNF α, and nitric oxide (NO) were measured. Results The serum levels of IFNα, IL-8, TNFα and NO in 58 normal controls were 22.74 ± 73.82ng / L, 13.24 ± 5.36ng / L, 217.14 ± 76.30ng / L respectively, 2 5 .98 ± 8.70 μmol / L. The levels of serum IFNα, IL 8, TNFα and NO in CMV antibody-positive infants were respectively 5 82.26 ± 131.72ng / L, 75.28 ± 33.5 7ng / L, 42 9.46 ± 15 6.32ng / L and 5 9.87 ± 16.42 μmol / L, respectively, which were significantly higher than those in the normal control group (P <0.01). The above four indexes in the group of HCV antibody-positive liver were 55.32 ± 114.64ng / L and 71.34 ± 2 7.64ng / L, 374.35 ± 138.4ng / L and 62.4 ± 2 1.38μmol / L, respectively, which were also significantly higher than those in the normal control group (P <0.001). There was no significant difference between CMV and HCV antibody-positive infant group (P> 0.05). TNFα and NO in the two groups of infants were positively correlated (r1 = 0.62, r2 = 0.57, P <0.01). Conclusions The serum levels of TNFα, IL8 and IFN in children with CMV and HCV antibody positive acute