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目的 分析急性白血病(急淋初治37例、缓解期32例，急非淋 9例）细胞凋亡量、DNA含量的变化及临床意义。方法 采用流式细胞术(FCM)碘化丙啶插入性DNA定量染色法。结果 凋亡百分率在缓解期最高，为19.92±8.05，初治组为8.16±3.12，与正常儿有显著性差异；白血病儿DNA指数（DI值）、异倍体（AN）率、SPF值（S期细胞百分比）均显著性增高，异倍体的发生与肝脾大小、白细胞数有关，与FAB分型无关。结论 小儿白血病细胞存在 DNA含量的异常，凋亡量、SPF等指标对提示预后有一定的参考价值。
Objective To analyze the changes of apoptosis and DNA content in acute leukemia (37 cases of acute leukemia in initial stage, 32 cases of remission, 9 cases of acute non-lymphocytic leukemia) and its clinical significance. Methods Flow Cytometry (FCM) DNA propidium iodide quantitative staining. Results The percentage of apoptotic cells in the remission stage was the highest (19.92 ± 8.05) and that of the untreated group (8.16 ± 3.12), which was significantly different from that of the normal children. The DNA index (DI), aneuploidy (AN) S phase cells) were significantly increased, aneuploidy occurred with the size of liver and spleen, white blood cell count, and FAB type has nothing to do. Conclusion Abnormal DNA content in children with leukemia cells, apoptosis, SPF and other indicators of the prognosis of a certain reference value.