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目的 探讨乙型肝炎病毒蛋白对核酶剪切作用的影响 .方法 利用计算机辅助设计针对乙型肝炎病毒 C基因的三个单一核酶构建 Rz1核酶自剪切转录载体 (p GEMRz1) ,观察单一核酶 (Rz1)对靶 RNA的切割作用及乙型肝炎病毒蛋白对核酶剪切作用的影响 .结果 构建的核酶自剪切转录载体体外转录后 ,在顺式核酶发生自剪切后可将目的核酶正确地释放出来 ,计算机设计的单一核酶体外可剪切靶 RNA;乙型肝炎病毒蛋白对核酶剪切的无明显的抑制或增强作用 ,进一步的结果未观察到乙型肝炎病毒蛋白和靶 RNA的相互作用 .结论 体外转录的核酶可剪切乙肝病毒靶 RNA,在本研究条件下未观察到乙肝病毒蛋白对核酶切割作用的影响 ,从而确保核酶在细胞内能有效发挥作用 .
Objective To investigate the effect of hepatitis B virus (HBV) protein on the cleavage of the ribozyme.Methods To construct the Rz1 ribozyme self-shearing transcription vector (p GEMRz1) by computer-aided design of three single ribozymes against hepatitis C virus C gene, The effect of ribozyme (Rz1) on the target RNA cleavage and the effect of hepatitis B virus (HBV) protein on the cleavage of ribozyme.Results The constructed ribozyme was transcribed from the transcribed vector in vitro and after the self-cleavage of cis-ribozyme The target ribozyme can be correctly released, and the single ribozyme designed by the computer can cut the target RNA in vitro; the hepatitis B virus protein has no obvious inhibition or enhancement effect on the ribozyme shearing. Further results are not observed in type B Hepatitis virus protein and target RNA.Conclusion In vitro transcription of ribozyme can cut hepatitis B virus target RNA in this study did not observe the hepatitis B virus protein ribozyme cleavage effect in order to ensure that the ribozyme in the cell Can effectively play a role.