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目的探讨传统免疫印迹法(IB)和线性免疫印迹法(LB)检测Sm抗体的结果差异。方法用IB和LB分别测定48例系统性红斑狼疮(SLE)和30例正常人血清中Sm抗体,评价两者的敏感性和特异性。结果IB和LB法检测SLE患者血清中Sm抗体的敏感性分别是47.9%和37.5%;两者的特异性分别是100.0%和96.7%。结论IB法检测SLE患者Sm抗体的敏感性高于LB法(P<0.05);而两者的特异性差异无统计学意义(P>0.05)。
Objective To investigate the differences of Sm antibodies detected by traditional immunoblotting (IB) and linear immunoblot (LB). Methods 48 cases of systemic lupus erythematosus (SLE) and 30 cases of normal human serum Sm antibody were determined by IB and LB respectively to evaluate their sensitivity and specificity. Results The sensitivity of IB and LB to the detection of Sm antibody in sera of SLE patients was 47.9% and 37.5%, respectively. The specificity of the two antibodies was 100.0% and 96.7% respectively. Conclusion The sensitivity of IB method to detect Sm antibody in patients with SLE is higher than that of LB method (P <0.05). There is no significant difference between the two methods (P> 0.05).