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本文用钙离子荧光探针Ｆｌｕｏ－３和ＰＥ－ＣＤ３双标记的方法，通过流式细胞计测定ＰＷＭ对ＣＤ３表达量不同的胎儿胸腺细胞内游离钙浓度的影响。结果表明，ＰＷＭ能迅速诱导胸腺细胞并且主要是ＣＤ３＋细胞钙动员，７０ｍｉｎ后胞内钙基本降至基线水平；Ｈ７、ＣＰＺ和ＥＧＴＡ均能抑制ＰＷＭ诱导的钙动员。
In this paper, the effects of PWM on the intracellular free calcium concentration in fetal thymocytes with different expression levels of CD3 were determined by flow cytometry with the double labeling method of Fluo-3 and PE-CD3. The results showed that PWM could rapidly induce thymus cells and mainly CD3 + cells calcium mobilization. After 70 minutes, intracellular calcium decreased to baseline level. Both H7, CPZ and EGTA could inhibit the calcium-induced calcium mobilization.