论文部分内容阅读
1983~1984年我们对新疆发生的大豆病毒病进行了毒源鉴定工作。病毒的寄主范围很窄,测定的十四种植物中只能侵染大豆和菜豆品种Topcrop,不能侵染菜豆其他品种、豇豆、蚕豆、绿豆、豌豆、眉豆等豆科植物,也不能侵染普通烟、曼陀罗、百日菊、苋色藜、昆诺阿藜等。病毒的种传率为0.6—15.2%,棉黑蚜、桃蚜可以传毒。病毒的致死温度为60℃,稀释限点1:1000,体外保毒期四天。所有毒源标样与标准的大豆花叶病毒(SMV)抗血清发生明显的沉淀反应,一少部分标样与SMV抗血清发生反应的同时,还与苜蓿花叶病毒(AMV)的抗血清发生反应。电镜上观察病毒粒体为线状,大小为700~800×10~15毫微米。根据以上性状,引致新疆大豆病毒病的毒原主要为大豆花叶病毒(SMV)。
From 1983 to 1984, we carried out the source identification of soybean virus disease in Xinjiang. The virus host range is very narrow, the determination of fourteen kinds of plants can only infect soybean and bean varieties Topcrop, can not infect other varieties of bean, cowpea, fava bean, mung bean, peas, black beans and other leguminous plants, can not be infected Ordinary tobacco, Mandala, Zinnia, Amaranthus veratris, Quinoa and so on. Virus breeding rate of 0.6-15.2%, cotton black aphid, peach aphid can poison. Lethal temperature of the virus is 60 ℃, dilution limit of 1: 1000, four days in vitro protection period. Significant precipitation reaction was observed between all the virus source samples and the standard SMV antiserum. A small proportion of standard samples reacted with SMV antiserum and antisera with alfalfa mosaic virus (AMV) reaction. The electron microscopic observation of virus particles is linear, the size of 700 ~ 800 × 10 ~ 15 nm. According to the above traits, the virulence of Xinjiang soybean virus disease is mainly soybean mosaic virus (SMV).