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【目的】将一株从阜康市食用菌栽培基地棉籽壳发酵料中的分离得到的芽孢杆菌FKB-1,依据形态学与16S r DNA序列比对明确其分类地位。【方法】采用对峙平板法进行初筛、复筛,然后利用抑菌圈法测定抑菌效果,并镜检菌丝生长情况。通过形态学观察,生理生化及16S r DNA基因序列对比分析研究其分类地位。【结果】生防细菌FKB-1对绿色木霉菌具有稳定的抑菌效果,抑菌直径率达57%;同时16S r DNA序列比对结果显示其为解淀粉芽孢杆菌。【结论】FKB-1是一株抗木霉菌的生防解淀粉芽孢杆菌。
【Objective】 Bacillus sp. FKB-1, isolated from the fermented material of cotton seed hulls in Fukang City, was identified according to the morphological and 16S r DNA sequence alignment. 【Method】 The initial screening and re-screening were carried out by the confrontation plate method, then the antibacterial effect was determined by the inhibition zone method, and the mycelial growth was examined microscopically. Morphological observation, physiological and biochemical and 16S r DNA sequence comparison analysis of its taxonomic status. 【Result】 The biocontrol bacterium FKB-1 had a stable antibacterial activity against Trichoderma viride and the antibacterial diameter was 57%. Meanwhile, the 16S r DNA sequence alignment showed that it was Bacillus amyloliquefaciens. 【Conclusion】 FKB-1 is an anti-Trichoderma strain against Bacillus amyloliquefaciens.