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利用real-time RT-PCR和高效液相色谱技术对水稻条纹病毒(Rice stripe virus,RSV)侵染水稻(Oryza sativa subsp.japonica)植株和水稻悬浮细胞内的生长素合成酶基因YUCAA1表达量和内源生长素含量的变化分别进行了测定。结果表明,在细胞水平,RSV侵染后的16～64 h内能显著引起YUCAA1 mRNA表达量的上调和内源生长素含量的升高。与同一生长阶段的健康水稻相比,水稻植株接种后4～8 d内也可导致YUCAA1 mRNA表达量的上调和内源生长素含量的上升,而在接种后12和16 d时,病株内的YUCAA1 mRNA的表达量和内源生长素的含量均下降。这表明在RSV侵染水稻后的发病过程中,RSV能够调控寄主植物内源生长素的合成。同时,利用KPSC缓冲液处理病株来消除其内源生长素,能够引起RSV CP基因表达上调近2.9倍,另外用30μmol/L IAA溶液处理病株可使其体内的RSV CP基因表达下调45%,表明水稻体内生长素含量的变化能够影响RSV在寄主体内的复制。
The expression of auxin synthase gene YUCAA1 in rice stripe virus (RSV) -infected rice (Oryza sativa subsp. Japonica) and rice suspension cells was analyzed by real-time RT-PCR and high performance liquid chromatography Changes in endogenous auxin content were measured. The results showed that at the cellular level, the expression of YUCAA1 mRNA and the increase of endogenous auxin content were significantly induced within 16-64 h after RSV infection. Compared with healthy rice at the same growth stage, rice plants also induced the up-regulation of YUCAA1 mRNA expression and the increase of endogenous auxin content within 4-8 days after inoculation, but at 12 and 16 days after inoculation, Of YUCAA1 mRNA expression and endogenous auxin content decreased. This indicates that RSV can regulate the synthesis of endogenous auxin in host plants during the pathogenesis after RSV infects rice. At the same time, treatment of diseased plants with KPSC buffer to eliminate endogenous auxin can cause the expression of RSV CP gene increased by nearly 2.9-fold. In addition, treatment of diseased plants with 30μmol / L IAA reduced the expression of RSV CP gene by 45% , Indicating that the change of auxin content in rice can affect the replication of RSV in the host.