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本研究以刺五加鲨烯环氧酶(squalene epoxidase,SE)基因的c DNA序列为基础设计特异性引物,利用PCR和TAIL-PCR扩增SE DNA的全长序列,结合Plant CARE等软件,对启动子中的作用元件进行生物信息学分析。克隆得到长6 307 bp的刺五加SE DNA和启动子序列,包含8段外显子、7段内含子及两端的非翻译区,编码554个氨基酸;上游启动子序列长1 907 bp,含36个TATA-box,27个CAAT-box,此外还有茉莉酸甲酯调控元件、脱落酸调控元件以及光调控元件等多种顺势作用元件,表明SE基因表达受植物激素、光照以及温度等多种因素的调控。
In this study, specific primers were designed based on the c DNA sequence of squalene epoxidase (SE) gene. The full length of SE DNA was amplified by PCR and TAIL-PCR, and combined with Plant CARE software, Bioinformatic analysis of the active elements in the promoter. A 6 307 bp long Acanthopanax senticosus SE DNA sequence and a promoter sequence were cloned, including 8 exons, 7 intron and untranslated region at both ends, encoding 554 amino acids. The upstream promoter sequence was 1 907 bp in length, Including 36 TATA-boxes and 27 CAAT-boxes, as well as homeopathic elements such as methyl jasmonate regulatory elements, abscisic acid regulatory elements and light regulatory elements, indicating that SE gene expression is affected by plant hormones, light and temperature Regulation of many factors.