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[目的]使用聚乳酸聚乙醇酸共聚物(PLGA)制备纳米颗粒,用来包载MCF7乳腺癌细胞的裂解蛋白,并初步评估其在乳腺癌免疫治疗中的作用。[方法]用二次乳化法制备载MCF7裂解蛋白的纳米粒子,对其包封效率、粒径、形态和Zeta电位进行测定,优化制备参数以提高包封效率;用该纳米粒子刺激人外周血单个核细胞,检测纳米粒子的体外抗肿瘤免疫效应。[结果]制备的载MCF7裂解蛋白纳米颗粒呈球形,尺寸较均匀,平均粒径75nm,表面带负电荷,对MCF7裂解蛋白的包裹率可达85%,并能显著提高人淋巴细胞对乳腺癌细胞的体外杀伤能力,约是对照组的3倍(P<0.05)。[结论]该制备方法所得载MCF7裂解蛋白纳米粒理化性质优良,包裹率高,在体外实验中可提高MCF7裂解蛋白的免疫原性,在提升抗肿瘤免疫效应方面具有应用潜能。
[Objective] The purpose of this study was to prepare nanoparticles with polylactic acid polyglycolic acid copolymer (PLGA) to encapsulate the cleavage proteins of MCF7 breast cancer cells and to evaluate their role in breast cancer immunotherapy. [Method] The nanoparticles containing MCF7 cleaved protein were prepared by secondary emulsification and the encapsulation efficiency, particle size, morphology and Zeta potential were determined, and the preparation parameters were optimized to improve the encapsulation efficiency. The nanoparticles were used to stimulate human peripheral blood Mononuclear cells, detecting the antitumor immune effect of nanoparticles in vitro. [Result] The prepared MCF7 lysis protein nanoparticles were spherical in shape and uniform in size with an average particle diameter of 75 nm. The surface was negatively charged, and the encapsulation rate of the MCF7 cleaved protein was 85%, and significantly enhanced the effect of human lymphocytes on breast cancer Cells in vitro cytotoxicity, about 3 times the control group (P <0.05). [Conclusion] The prepared MCF7 lysed protein nanoparticles have good physico-chemical properties and high encapsulation efficiency, and can improve the immunogenicity of the MCF7 cleaved protein in vitro and have potential application in enhancing anti-tumor immune effect.