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目的:课题组前期实验发现人脐血源基质细胞(hUCBDSC)体外具有促进造血细胞集落扩增的能力,文中拟采用CM-DiI荧光标记技术,观察hUCBDSC移植后的归巢、定位和增殖情况。方法:传代培养hUCBDSC,CM-DiI荧光染料预染后经尾静脉输入BALB/c-nu/nu裸鼠体内,分别于移植后1、7、14、21 d取裸鼠骨髓、脾脏、肝脏、肺脏组织,激光共聚焦显微镜观察CM-DiI标记hUCBDSC体内分布情况。结果:传代培养hUCBDSC呈成纤维样,CM-DiI染色后胞膜呈红色。经尾静脉移植至裸鼠体内,移植后1 d,hUCBDSC广泛分布在骨髓、脾脏、肝脏、肺脏等组织中,移植7 d以后,hUCBDSC主要分布在骨髓,在骨髓中增殖、分化;脾脏、肝脏、肺脏等组织中的hUCBDSC明显减少。结论:人脐血源基质细胞经尾静脉输注可“归巢”至骨髓,并在骨髓中增殖、分化,重建受损造血微环境。
OBJECTIVE: In our previous study, human umbilical cord blood-derived stromal cells (hUCBDSCs) were found to promote the proliferation of hematopoietic cells in vitro. CM-DiI fluorescent labeling was used to observe the homing, localization and proliferation of hUCBDSC after transplantation. Methods: After subcultured with hUCBDSC and CM-DiI fluorescent dye, BALB / c nude nude mice were transplanted via caudal vein. The nude mouse bone marrow, spleen and liver were harvested at 1, 7, 14 and 21 days after transplantation respectively. Lung tissue, confocal laser scanning microscopy CM-DiI labeled hUCBDSC in vivo distribution. Results: The subculture hUCBDSC fibroblast-like, CM-DiI staining red membrane. HUCBDSC was widely distributed in bone marrow, spleen, liver and lung tissues at 1 d after transplantation. After transplantation for 7 days, hUCBDSC mainly distributed in bone marrow and proliferated and differentiated in bone marrow. Spleen and liver , HUCBDSC in lungs and other tissues was significantly reduced. CONCLUSION: Human umbilical cord blood-derived stromal cells can be “homing” into the bone marrow via tail vein infusion and proliferate and differentiate in the bone marrow to reconstruct the damaged hematopoietic microenvironment.