AIM:To construct pEGFP-hepatocyte growth factor(HGF)expression vector,the to detect its expression in transfectedhuman hepatocytes,and to investigate the influence ofautocrine HGF expression on the proliferative potential andcytoprotective effects in human hepatocytes.METHODS:Human HGF cDNA was ligated to the pEGFP vector.Recombinant plasmid was transfected into human hepatocyteline QZG wibh liposome.Expression of HGF protein was observedby fluorescence microscopy and immunohistochemistry.Hepaticcells were collected 24,48,and 72 h after transfection todetect the number of[~3H]-TdR uptake in DNA.DNA synthesiswas observed by using PCNA stain immunohistochemistry.Acute liver cell damage was induced by carbontetrachloride.Cytoprotective effect was observed byexamining the survival rate of hepatocytes and leakage ofintracellular alanine transaminase(ALT)and potassium ions.RESULTS:HGF identification of pEGFP-HGF by enzymedigestion showed that HGF fragment was cloned into BamHI and Sa/I sites of pEGFP-N3.Expression of GFP in transfectedhepatocytes was observed with fluorescence microscopy.The[~3H]-TdR uptake became 7 times as many as in thecontrol group 96 h after transfection.After HGF transfection,the survival rate of hepatocytes poisoned by CCI_4 significantlyincreased(83% vs 61%,P<0.05),and the leakage ofintracellular alanine transaminase and potassium ions decreased(586 nkat/L vs 1089 nkat/L,P<0.01;and 5.59 mmol/L vs6.02 mmol/L,P<0.01 respectively).Culture of transfectedhepatic cells promoted the proliferation of other non-transfected cells.CONCLUSION:Transfected HGF is expressed in hepaticceils and has the activity of promoting cell division andprotecting hepatic cells against poisoning. AIM: To construct pEGFP-hepatocyte growth factor (HGF) expression vector, the to detect its expression in transfected human hepatocytes, and to investigate the influence of autoocrine HGF expression on the proliferative potential and cytoprotective effects in human hepatocytes. METHODS: Human HGF cDNA was ligated to the pEGFP vector.Recombinant plasmid was transfected into human hepatocyteline QZG wibh liposome. Expression of HGF protein was observed by fluorescence microscopy and immunohistochemistry. Hepatocytes were collected 24, 48, and 72 h after transfection to detect the number of [~ 3H] -TdR uptake in DNA.DNA synthesis was observed by using PCNA stain immunohistochemistry. Acute liver cell damage was induced by carbontetrachloride. Cytoprotective effect was observed by the survival rate of hepatocytes and leakage of intracellular alanine transaminase (ALT) and potassium ions .RESULTS: HGF identification of pEGFP-HGF by enzymediugression showed that HGF fragment was cloned into BamHI and Sa / I sites of pEGFP-N3.Expression of GFP in transfected hepatocytes was observed with fluorescence microscopy.The [~ 3H] -TdR uptake became 7 times as many as in ascontrol group 96 h after transfection. After HGF transfection, the survival rate of hepatocytes poisoned by CCI_4 significantly increased (83% vs 61%, P <0.05), and the leakage ofintracellular alanine transaminase and potassium ions decreased (586 nkat / L vs 1089 nkat / L, P <0.01; and 5.59 mmol / L vs6.02 mmol / P <0.01 respectively). Culture of transfected hepatic cells promoted the proliferation of other non-transfected cells. CONCLUSION: Transfected HGF is expressed in hepatic cells and has the activity of promoting cell division and protecting hepatic cells against poisoning.