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HBsAg阳性人肝细胞瘤细胞系huH-4含有单一5.6kb HBV DNA整合序列,现已将它克隆到柯奇载体pHC79.由于多重排,只有开架阅读框(orf)X和pre S/S基因仍保持功能状态.为检测由整合的HBV DNA编码的可能的反式激活活性,作者构
The HBsAg-positive human hepatoma cell line huH-4 contains a single 5.6 kb HBV DNA integration sequence that has been cloned into the Koach vector pHC79. Due to the multiple rearrangements, only the open reading frame (orf) X and pre S / S genes are still Maintaining Functional Status To test for possible transactivation activity encoded by the integrated HBV DNA,