论文部分内容阅读
目的探讨主要组织相容性复合体Ⅰ类相关基因A(MICA)对胰腺癌诊断的临床意义。方法 PCR检测41例胰腺癌组织及癌旁组织MICA mRNA表达情况;ELISA检测56例胰腺癌、65例慢性胰腺炎和81例正常体检者血清中MICA浓度,并分析其与临床病理参数相关性。结果 MICA mRNA在胰腺癌组织表达阳性率和相对表达量显著高于癌旁组织,差异有统计学意义(P<0.05);Ⅰ期+Ⅱ期表达显著高于Ⅲ期+Ⅳ期,高分化者显著高于低分化者表达量;胰腺癌患者血清中MICA浓度明显高于慢性胰腺炎组和正常对照组水平,Ⅲ期+Ⅳ期血清中MICA浓度明显高于Ⅰ期+Ⅱ期,淋巴结转移者血清浓度显著高于未转移者。结论早期分化较好的胰腺癌组织细胞MICA表达量较高,迅速诱导NK细胞杀伤肿瘤细胞发挥免疫监视作用;晚期肿瘤细胞表面MICA蛋白脱落释放到间质及血清中形成可溶性MICA进而引起肿瘤免疫逃逸。
Objective To investigate the clinical significance of the major histocompatibility complex class Ⅰ related gene A (MICA) in the diagnosis of pancreatic cancer. Methods MICA mRNA expression was detected by PCR in 41 cases of pancreatic cancer tissues and paracancerous tissues. MICA concentration was detected by ELISA in 56 cases of pancreatic cancer, 65 cases of chronic pancreatitis and 81 cases of normal controls. The correlation between MICA mRNA and clinicopathological parameters was analyzed. Results The positive rate and relative expression of MICA mRNA in pancreatic cancer tissues were significantly higher than those in paracancerous tissues (P <0.05). The expression of MICA mRNA in stage Ⅰ and stage Ⅱ was significantly higher than that in stage Ⅲ and Ⅳ, The level of MICA in serum of patients with pancreatic cancer was significantly higher than that of patients with chronic pancreatitis and normal control. The level of MICA in patients with stage Ⅲ + Ⅳ was significantly higher than that in patients with stage Ⅰ + Ⅱ, and those with lymph node metastasis Serum concentrations were significantly higher than those without metastasis. Conclusions The expression of MICA in early well differentiated pancreatic cancer cells is high, which can induce NK cells to kill tumor cells rapidly. The MICA protein on the surface of advanced cancer cells released into the interstitial and serum to form soluble MICA and then caused tumor immune escape .