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本文应用ELISA间接法检测60例下呼吸道感染患儿的耳血呼吸道合胞病毒(RSV)IgM抗体;以病毒分离和/或补体结合试验作对照,其诊断符合率为88.3％,同步检测静脉血清RSV IgM抗体,其计数资料相关分析和定量相关回归分析均有高度显著性差异(P<0.01).现报道如下. 对象与方法 一、对象:1987～1988年冬、春,本院儿科住院的60例急性下呼吸道感染患儿,于入院时耳垂采血20μl.将血滴在新华定量滤纸上。采集耳血的同时取静脉血1.5ml。分离血清。 二、方法:滤纸干滴血于检测前一晚剪下,浸泡在0.5ml10％BSA—PBS—T20稀释液中4℃过夜。血清于检测当日用上述溶液按1:50稀释。操作步骤如下。用RSV纯化抗原包被反应板,设正常抗原
In this study, 60 cases of ear-respiratory syncytial virus (RSV) IgM antibodies in children with lower respiratory tract infection were detected by ELISA indirect method. The diagnostic coincidence rate was 88.3% by virus isolation and / or complement fixation test. Synchronous detection of venous serum RSV IgM antibody, count data analysis and quantitative correlation analysis showed a highly significant difference (P <0.01), are reported as follows.Objects and methods First, the object: 1987 ~ 1988 winter, spring, hospital pediatric hospital 60 cases of acute lower respiratory tract infection in children, admitted to the earlobe blood 20μl blood will drop in Xinhua quantitative filter paper. Blood collected at the same time take the blood 1.5ml. Separation of serum. Second, the method: Drip filter paper cut in the night before the test, soaked in 0.5ml10% BSA-PBS-T20 dilution at 4 ℃ overnight. Serum was diluted 1:50 with the above solution on the day of testing. The operation steps are as follows. The antigen plate was coated with RSV purified antigen and the normal antigen was set