论文部分内容阅读
采用逐步提高ＮａＣｌ浓度的方法，获得了耐１％ＮａＣｌ的烟草（ＮｉｃｏｔｉａｎａｒｉｓｔｉｃａＬ．ｃｖＧａｎｓｕＹｅｌｌｏｗ一ｆｌｏｗｅｒ）愈伤组织变异系（Ｓ＿１）。与原始型愈伤组织（Ｓ＿０）相比，在１％ＮａＣｌ胁迫下，Ｓ＿１的干重增量高于Ｓ＿０４．６倍，组织渗透势下降，而含水量提高；游离脯氨酸、蔗糖、还原糖含量及乙烯释放速率的增加Ｓ＿０高于Ｓ＿１，但腐胺的累积量相反。Ｓ＿１对甘露醇（Ｍａｎｉｔｏｌ）渗透胁迫和Ｎａ＿２ＳＯ＿４盐胁迫也具有适应性，但对低温（０一１℃）敏感。ＡＢＡ促进了Ｓ＿０对盐胁迫的抗性，但对已耐盐的Ｓ＿１无类似作用。
With the method of increasing the NaCl concentration step by step, the mutant (S 1) of the callus of tobacco (Nicotiana farica L. cv Gansu Yellow Flower) resistant to 1% NaCl was obtained. Compared with the original callus (S_0), under the stress of 1% NaCl, the dry weight increment of S_1 was higher than that of S_04.6, the osmotic potential of tissue was decreased and the water content was increased. The content of free proline, sucrose, The increase of sugar content and ethylene release rate S_0 was higher than that of S_1, but the accumulation of putrescine was opposite. S_1 is also suitable for Manitol osmotic stress and Na_2SO_4 salt stress, but sensitive to low temperature (0 to 1 ℃). ABA promoted the resistance of S 0 to salt stress, but had no similar effect on salt-tolerant S 1.