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目的:利用显微注射方法产生整合庚型肝炎病毒(HGV)全基因的转基因小鼠,并研究HGV基因的遗传特性。方法:将含有HGV全长基因的载体线性化后,将目的基因DNA溶于显微注射用缓冲液,依常规方法进行显微注射,得到仔鼠后用PCR及基因组DNA Southern印迹法鉴定。阳性小鼠即为建立者(founder)小鼠,将founder小鼠与正常小鼠交配得到F代小鼠,随后将F1代阳性鼠与正常小鼠交配得到F2代小鼠。结果:共得到5只founder小鼠,其中4只与正常小鼠交配,得到F1代小鼠共41只,其中29只为阳性,阳性率为71％。F2代小鼠共21只,其中16只为阳性,阳性率为76％。结论:得到了整合有HGV全长基因的转基因小鼠,并证明HGV基因可以在转基因小鼠体内稳定传递。
OBJECTIVE: To generate transgenic mice integrating the gene of hepatitis G virus (HGV) by microinjection and to study the genetic characteristics of HGV gene. Methods: The vector containing HGV full-length gene was linearized. The target gene DNA was dissolved in microinjection buffer and microinjected according to the conventional method. The offspring were identified by PCR and genomic DNA Southern blotting. The positive mice are founder mice, the founder mice are mated with normal mice to obtain F generation mice, and the F1 generation mice are then mated with normal mice to obtain F2 generation mice. RESULTS: A total of 5 founder mice were obtained, of which 4 were mated with normal mice, and 41 F1 mice were obtained, of which 29 were positive and the positive rate was 71%. Twenty-one F2 mice were positive, of which 16 were positive and the positive rate was 76%. Conclusion: The transgenic mice with the full-length HGV gene were obtained and proved that the HGV gene can be stably transmitted in the transgenic mice.