本法基于只有活的细菌能合成DNA并渗入~3H-胸腺嘧啶核苷(TdR),将吞噬细胞和细菌一起孵育,然后通过一次冰冻融化过程(freeze-thawCycle)破坏吞噬细胞。根据测定的存活微生物吸取~3H-TdR的量来估计吞噬细胞的杀菌功能。本法简单、敏感、用血量少,适宜作白细胞功能的常规试验。细菌悬液的制备将金黄色葡萄球菌种在液体增菌培养基中过夜培养,用磷酸盐缓冲液洗细菌二 This method is based on the fact that only viable bacteria can synthesize DNA and penetrate into ~ 3H-thymidine (TdR), incubate phagocytes and bacteria, and then destroy phagocytes by a freeze-thaw cycle. The bactericidal function of phagocytes was estimated from the measured amount of ~ 3H-TdR taken by viable microorganisms. This method is simple, sensitive, with less blood, suitable for routine testing of white blood cell function. Preparation of Bacterial Suspension Staphylococcus aureus species were cultured overnight in liquid enrichment medium and bacteria were washed with phosphate buffer