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制备破伤风类毒素阳离子葡聚糖微球,并对微球的载药机制进行考察。采用乳化交联法在双水相中制备了荷正电的甲基丙烯酸羟乙酯-葡聚糖微球,并基于静电作用原理后载破伤风类毒素疫苗。微球的zeta电位随甲基丙烯酸二甲氨基乙酯加入量的增加而增大,激光共聚焦显微实验表明,异硫氰酸荧光素牛血清可以渗透进入阳离子葡聚糖微球的内部,而不能进入中性葡聚糖微球。采用后载法制备的破伤风类毒素阳离子葡聚糖微球的包封率显著高于前载法。改变甲基丙烯酸羟乙酯-葡聚糖的取代度可以调控阳离子葡聚糖微球的体外释放行为。
Tetanus toxoid cationic dextran microspheres were prepared, and the microspheres drug loading mechanism were investigated. Hydroxylethyl methacrylate-dextran microspheres were prepared by emulsion cross-linking method in water-in-water phase and tetanus toxoid vaccine was loaded based on the principle of electrostatic interaction. The zeta potential of the microspheres increased with the increase of the amount of dimethylaminoethyl methacrylate. Confocal laser microscopy showed that fluorescein isothiocyanate (BIS) could penetrate into the interior of the cationic dextran microspheres, And can not enter the neutral dextran microspheres. The entrapment efficiency of tetanus toxoid cationic dextran microspheres prepared by the post-loading method was significantly higher than that of the former method. Changing the degree of substitution of hydroxyethyl methacrylate-dextran can regulate the release behavior of cationic dextran microspheres in vitro.