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目的观察水杨酸钠对胰岛素抵抗大鼠肝脏胰岛素敏感性的影响。方法大鼠随机分为3组:分别给大鼠静脉输注脂肪乳(脂肪乳组),脂肪乳+水杨酸钠(实验组)和生理盐水组,并在输注后2 h,进行清醒状态下扩展高胰岛素-正血糖钳夹术,检测肝脏组织中NF-κB及IL-6、TNF-α、SOCS-3 mRNA的表达。结果脂肪乳组大鼠钳夹稳态下,内生葡萄糖产物(EGP)较基础状态降低比率仅是生理盐水组大鼠的38%,水杨酸钠提高EGP降低幅度明显(P<0.01),改善了肝脏胰岛素敏感性。脂肪乳组肝细胞核NF-κB表达量是生理盐水组2.4倍,肝组织中IL-6、TNF-α及SOCS-3 mRNA表达较生理盐水组升高,输注水杨酸钠使核内NF-κB较输注脂肪乳下降40%,炎症因子表达相应降低(P<0.01)。结论水杨酸钠可抑制肝脏组织NF-κB活化,进而降低炎症因子蓄积,发挥改善肝脏胰岛素抵抗的作用。
Objective To observe the effect of sodium salicylate on hepatic insulin sensitivity in insulin resistant rats. Methods Rats were randomly divided into 3 groups: the rats were given intravenous infusion of fat emulsion (fat emulsion group), fat emulsion + sodium salicylate (experimental group) and saline group, respectively. After 2 h infusion, State extended insulin high blood glucose clamp, detection of liver tissue NF-κB and IL-6, TNF-α, SOCS-3 mRNA expression. Results Compared with the rats in normal saline group, the reduction rate of endogenous glucose product (EGP) was only 38% in rats in the lipid emulsion group, while the reduction of EGP by sodium salicylate was significant (P <0.01) Improve liver insulin sensitivity. The expression of NF-κB in fatty liver group was 2.4-fold higher than that in normal saline group. The expression of IL-6, TNF-α and SOCS-3 mRNA in liver tissue were higher than those in normal saline group. Sodium salicylate infused NF -κB was 40% lower than that of trans fat emulsion, and the expression of inflammatory cytokines decreased accordingly (P <0.01). Conclusion Sodium salicylate can inhibit the activation of NF-κB in liver tissue, and then reduce the accumulation of inflammatory cytokines and play an important role in improving hepatic insulin resistance.