[Objective] This study aimed to clone and analyze the cysteine proteinase inhibitor gene from seedless litchi. [Method] According to the EST sequence of cysteine proteinase inhibitor in constructed SSH subtractive library of seedless litchi abortion, nucleotide sequence of the cysteine proteinase inhibitor gene was obtained by using RACE technology and analyzed by using bioinformatics software. [Result] A cysteine protease inhibitor gene was obtained with the sequence of 635 bp containing a 321 bp open reading frame. It was predicted that the encoded protein contained 106 amino acids with conserved domain of cysteine proteinase inhibitor and had relatively high homology with the cysteine proteinase inhibitor gene of several species. [Conclusion] This study laid the foundation for further exploring the physiological functions of this cysteine proteinase inhibitor gene in plants. [Objective] This study aimed to clone and analyze the cysteine ​​proteinase inhibitor gene from seedless litchi. [Method] According to the EST sequence of cysteine ​​proteinase inhibitor in constructed SSH subtractive library of seedless litchi abortion, nucleotide sequence of the cysteine ​​proteinase inhibitor gene was obtained by using RACE technology and analyzed by using bioinformatics software. [Result] A cysteine ​​protease inhibitor gene was obtained with the sequence of 635 bp containing a 321 bp open reading frame. It was predicted that the encoded protein contained 106 amino acids with conserved domain of cysteine ​​proteinase inhibitor and had relatively high homology with the cysteine ​​proteinase inhibitor gene of several species. [Conclusion] This study laid the foundation for further exploring the physiological functions of this cysteine ​​proteinase inhibitor gene in plants.