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目的观察肝硬变大鼠血清及食管组织中一氧化氮（ＮＯ）含量及血流动力学改变．方法用６０％ＣＣｌ４油性溶液皮下注射，诱导肝硬变大鼠模型．随机取８只肝硬变大鼠作为实验组，另取８只正常ＳＤ大鼠作为对照组．应用５７Ｃｏ同位素标记微球技术测定肝硬变大鼠平均动脉压（ＭＡＰ）、门静脉压（ＰＶＰ）、心输出量（ＣＯ）、心脏指数（ＣＩ）、内脏血管阻力（ＳＶＲ）及内脏器官血流量（ＳＢＦ）；应用荧光比色法检测肝硬变大鼠血清及食管组织匀浆中ＮＯ含量．结果肝硬变大鼠血清（μｍｏｌ／Ｌ）及食管组织中ＮＯ含量（ｎｍｏｌ／ｇ）显著高于对照组（４２０４±１２５３对０５３２±０２５７，Ｐ＜００１；０２０３±００３２３对００６４±００１３５，Ｐ＜００１）；肝硬变大鼠全部出现高动力循环状态，其ＭＡＰ（ｋＰａ），１００ｇ体重的ＳＶＲ（ｋＰａ·ｍｌ－１·ｍｉｎ－１）显著低于对照组（１４４２±０４７对１７０５±０３４，Ｐ＜００１；２９７４±０１８６对４２３４±０１１８，Ｐ＜００１）；而ＰＶＰ，ＣＩ，ＣＯ，ＳＢＦ则显著高于对照组（Ｐ＜００１）．结论肝硬变大鼠存在高动力循环状态；内源性ＮＯ在肝硬变大鼠血流动力学改变中可能?
Objective To observe the changes of nitric oxide (NO) and hemodynamics in serum and esophageal tissues of cirrhotic rats. Methods A rat model of cirrhosis was induced by subcutaneous injection of 60% CCl4 oily solution. Eight rats with cirrhosis were randomly selected as experimental group and eight normal SD rats as control group. The mean arterial pressure (MAP), portal pressure (PVP), cardiac output (CO), cardiac index (CI), visceral vascular resistance (SVR) and blood flow of visceral organs in cirrhotic rats were measured by 57Co isotope labeled microspheres (SBF). The content of NO in serum and esophageal homogenate of rats with cirrhosis was detected by fluorescence colorimetry. Results The levels of NO (nmol / L) and esophageal NO (4/204 ± 1.253 vs 0532 ± 0257, P <001 ; 0203 ± 00323 vs 0064 ± 00135, P <001); all rats with cirrhosis showed hyperdynamic circulatory state with MAP (kPa), SVR of 100g body weight (kPa · ml -1 · min-1) was significantly lower than the control group (1442 ± 047 vs 1705 ± 034, P <001; 2974 ± 0186 vs 4234 ± 0.118 , P <001), while PVP, CI, CO, SBF were significantly higher than those in the control group (P <001). Conclusion There is a hyperdynamic circulatory state in cirrhotic rats. It is possible that endogenous nitric oxide may play a role in hemodynamic changes in cirrhotic rats.