利用体视显微镜和组织细胞分析系统软件对北五味子种子萌发过程中形态学进行动态观察,并对其部分生理生化指标进行分析。实验结果表明,层积处理(4℃,沙培)初期,种子胚部较小,富含油脂;处理后期,胚部变得细长,逐渐伸向种子尖端一侧。处理60 d时,种子开始萌动;至150 d时,种胚突破种皮限制,开始发芽。随着种胚的发育,种子的含水量和可溶性蛋白的含量增加,α-淀粉酶活性逐渐提高,而可溶性总糖的含量则呈现先降低而后增高的趋势。 The morphological changes of Schisandra sphenanthera seeds during germination were observed with stereo microscope and tissue cell analysis system software, and some physiological and biochemical indexes were analyzed. The experimental results showed that in the initial stage of stratification treatment (4 ℃, Sapi), the embryo of the seed was smaller and rich in oil. At the later stage of treatment, the embryo became slender and gradually extended to the tip of the seed. At 60 days, seeds began to germinate; to 150 days, embryos broke the seed coat and began to germinate. With the development of embryo, the content of water and soluble protein in seeds increased, the activity of α-amylase increased gradually, while the content of soluble sugar decreased first and then increased.