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Many viral epitope specific T cell receptors (TCRs) in MHC-matched individuals have been demonstrated to involve conserved amino acid motifs in β chain complementarity-determining region 3 (CDR3). However, it is not sure whether the conserved motifs can also be found in TCR α chain. In previous studies, we developed a modified method to enlarge the percentage of cytomegalovirus (CMV) pp65 peptide-specific CD8+ T cells in PBMC by continuous peptide stimulation in vitro, which provides sufficient number of specific T cells for detection. In this study, we further analyzed the restrictive usage of TCR Vα and Vβ gene families and investigated the CDR3 gene sequence of pp65 peptide-specific CD8+ T cells. Analysis of CDR3 spectratypes suggested a restricted usage of TCR α chain AV8, AV12, AV21, AV31 families and TCR β chain BV3, BV14, BV21, BV23, BV11 families in donor CD8+ T cells stimulated by pp65 peptide. The sequences of these T cells involved similar sequence (TX) G (X) A in CDR3 region of TCR α chain and L (XT) G (X) A in TCR β chain.
Many viral epitope specific T cell receptors (TCRs) in MHC-matched individuals have been demonstrated to contain conserved amino acid motifs in beta chain complementarity-determining region 3 (CDR3). However, it is not sure whether the conserved motifs can also be found in TCR α chain. previous developed, we developed a modified method to enlarge the percentage of cytomegalovirus (CMV) pp65 peptide-specific CD8 + T cells in PBMC by continuous peptide stimulation in vitro, which provides sufficient number of specific T cells for detection. Analysis of CDR3 spectratypes suggested a restricted usage of TCR α chain AV8, AV12, AV21, The sequences of these T cells are similar to (TX) G (X) A in CDR3, BV14, BV21, BV23, BV11 families in donor CD8 + T cells stimulated by pp65 peptide region of TCR α chain and L (XT) G (X) A in TCR β chain.