论文部分内容阅读
AIM: To study the deletion of mitochondiral DNA inhepatocellular carcinoma and hepatocellular nodularhyperplasia and its significance in the development of cancer.METHODS: Deleted mtDNA (CD-mtDNA) and wild typemtDNA (WT-mtDNA) were quantitatively analyzed byusing real-time PCR in 27 hepatocellular carcinomas (HCC)and corresponding noncancerous liver tissues and 27hepatocellular nodular hyperplasiae (HNH).RESULTS: A novel CD (4 981 bp) was detected in 85%(23/27) and 83%(22/27) of HCC and HNH tumor tissues,respectively,which were significantly higher than that inpaired noncancerous liver tissues (57%,15/27) (P<0.05).The CD/WT-mtDNA ratio in HCC tumors was 0.00092(median,interquartile range,0.0001202-0.00105),whichwas significantly higher than that in paired noncancerousliver tissues (median,0.000,quartile range,0-0) (P=0.002,Mann-Whitney Test),and was 25 of times of that in HNHtissues (median,0.0000374,quartile range,0-0.0004225)(P=-0.002,Mann-Whitney test).CONCLUSION: CD-mtDNA mutation plays an importantrole in the development and progression of HCC.
AIM: To study the deletion of mitochondrial DNA in hepatocellular carcinoma and hepatocellular nodular hyperplasia and its significance in the development of cancer. METHODS: Deleted mtDNA (CD-mtDNA) and wild type mtDNA (WT-mtDNA) were quantitatively analyzed by using real- time PCR in 27 The HCC and HNH (HNH) .RESULTS: A novel CD (4 981 bp) was detected in 85% (23/27) and 83% (22/27) of HCC and HNH tumor tissues, respectively, which were significantly higher than those inpaired noncancerous liver tissues (57%, 15/27) (P <0.05). The CD / WT-mtDNA ratio in HCC tumors was 0.00092 (median, interquartile range, 0.0001202-0.00105 ), which was significantly higher than that in paired noncancerousliver tissues (median, 0.000, quartile range, 0-0) (P = 0.002, Mann-Whitney Test), and was 25 times of that in HNHtissues (median, 0.0000374, quartile range , 0-0.0004225) (P = -0.002, Mann-Whitney test) .CONCLUSION: CD-mtDNA mutation plays an importantrole in the development and progression of HCC.