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以苏玉16(JB×Y53)的F2∶3家系为试验材料,选用分布在玉米10条染色体上的556对SSR引物,对玉米农艺性状进行基因定位并分析其遗传效应。结果表明,556对SSR引物对亲本Y53、JB及其F1进行多态性检测,获得85对多态性引物,多态率为15.3%,其中有76对引物扩增带型清晰,可用于后续QTL的研究工作。共检测到3个与抽穗期QTL连锁的标记,可解释表型变异的8.16%～14.10%;6个与株高QTL连锁的标记,可解释表型变异的6.01%～15.83%;6个与穗位高QTL连锁的标记,可解释表型变异的9.58%～31.89%;4个与茎粗QTL连锁的标记,可解释表型变异的5.84%～11.05%;2个与雄穗分枝数QTL连锁的标记,可解释表型变异的13.21%～24.76%;2个与雄穗长QTL连锁的标记,可解释表型变异的7.56%～7.67%;2个与散粉期QTL连锁的标记,可解释表型变异的7.14%～16.72%。
Using F2: 3 pedigree from Suyu 16 (JB × Y53) as test material, 556 pairs of SSR primers distributed on 10 chromosomes of maize were used to locate and analyze the genetic effects of agronomic traits in maize. The results showed that 556 pairs of SSR primers were used to detect the polymorphisms of Y53, JB and F1. 85 pairs of polymorphic primers were obtained with a polymorphic rate of 15.3%. Among them, 76 pairs of primers amplified bands clearly and could be used for subsequent QTL research work. A total of 3 markers linked to QTLs at heading stage were detected, accounting for 8.16% -14.10% of the phenotypic variance. Six markers linked with QTLs of plant height explained 6.01% -15.83% of phenotypic variation, The marker of high QTL linked to ear height could account for 9.58% ~ 31.89% of the phenotypic variance. Four markers linked with the QTL of QTL could account for 5.84% ~ 11.05% of the phenotypic variance. QTL linked to the marker explained 13.21% ~ 24.76% of the phenotypic variation; 2 markers linked to the long tassel QTL explained 7.56% ~ 7.67% of the phenotypic variance; 2 markers linked to the QTL for the loose powder stage, Can explain the phenotypic variation of 7.14% ~ 16.72%.