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应用Burgess等介绍的方法制备人胎盘的克隆形成刺激因子(CSF)(方法见Blood49:573,1977),对正常人及各种血液病患者的骨髓细胞进行单层软琼脂培养,观察加入CSF后对粒-巨噬细胞克隆形成能力的影响,并与沿用的双层软琼脂法的结果进行了比较。结果培养后4天开始出现CSF对粒-巨噬细胞克隆形成的刺激。随着培养天数的增加,克隆逐渐增大,6～7天达到高峰,以后则减低。形成的克隆数和
The placenta of human placenta was stimulated by the methods described in Burgess et al. (Methods Blood49: 573, 1977). Single-stranded soft agar culture was performed on bone marrow cells of normal people and various hematological patients. After addition of CSF On the ability of granulocyte macrophage colony formation, and compared with the results of the double-layer soft agar method. Results Stimulation of CSF on granulocyte-macrophage colony formation began 4 days after culture. Clones increased gradually with the increase of days of cultivation, reached the peak in 6 ~ 7 days and decreased later. Number of clones formed