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碱性橙Ⅱ和碱性嫩黄O均为工业用着色剂,不能作为食品添加用色素。为此建立了鱼肉样品中同时检查碱性橙Ⅱ和碱性嫩黄O的高效液相色谱定量分析方法,以甲醇-0.03%磷酸水溶液(pH 2.0)为流动相,Hydrosphere-C18色谱柱分离,二极管阵列检测器检测,检测波长为440 nm,可以实现鱼肉样品中添加的碱性橙Ⅱ和碱性嫩黄O的含量分析。该方法碱性橙Ⅱ和碱性嫩黄O的线性范围均为0.1～10μg/mL,最低检出浓度均为0.04μg/mL,方法的回收率为95%～116%,相对标准偏差(RSD)为2.0%～3.5%。此外,还进一步考察了前处理方法中采用磷酸-甲醇直接研磨鱼肉并抽提被分析物,抽提液适当蒸发浓缩,可以实现鱼肉样品中添加0.25μg/g的碱性橙Ⅱ和碱性嫩黄O的分析检测,以及自制浸泡黄鱼样品中的碱性橙Ⅱ和碱性嫩黄O的分析检测。
Both alkaline orange II and basic yellow O are industrial colorants, can not be used as food additives pigment. A high performance liquid chromatography (RP-HPLC) method for the simultaneous determination of basic orange II and alkaline yellow O was established in fish samples. The separation was performed on a Hydrosphere-C18 column with methanol-0.03% phosphoric acid solution (pH 2.0) Array detector detection, detection wavelength of 440 nm, can be achieved in fish samples added basic orange Ⅱ and alkaline yellow O content analysis. The linear range of this method was 0.1 ~ 10μg / mL and the lowest detection limit was 0.04μg / mL. The recoveries were between 95% and 116%. The relative standard deviations (RSDs) Is 2.0% ~ 3.5%. In addition, further study of the pretreatment method using phosphoric acid - methanol direct grinding fish and extract the analyte, the extract was concentrated by evaporation, the fish sample can be added 0.25μg / g alkaline orange Ⅱ and alkaline yellow O analysis of detection, as well as homemade soaked yellow croaker samples of alkaline orange Ⅱ and alkaline yellow O analysis and detection.