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目的了解急性呼吸道感染住院患儿中不同基因型人鼻病毒(Human Rhinovirus,HRV)流行情况,并进行遗传变异分析。方法采集2010年1月至2014年2月年成都某妇女儿童医院因急性呼吸道感染住院患儿的鼻咽抽提物1780份,采用巢式PCR方法,先用HRV-5’NCR引物筛检标本,了解该病毒总检出率,阳性标本再用HRV-VP4/VP2引物进行分型检测,利用MEGA6.06软件分析其变异情况。结果在1 780份鼻咽抽吸物标本中,HRV阳性为365例(20.5%,365/1 780),其中244份VP4/VP2基因阳性,经序列分析发现132例为A型、30例为B型、82例为C型;不同基因型的检出率在月份和年龄上有一定波动,其中A型和C型呈交互流行特点;经同源性分析发现不同基因型内和型间的核苷酸同源性差异较大。结论 HRV是成都地区急性呼吸道感染较为常见的病原之一,以HRV-A型和C型流行为主,各个基因型间具有较高的变异性。
Objective To understand the prevalence of different genotypes of human rhinovirus (HRV) in hospitalized children with acute respiratory infection and to analyze the genetic variation. Methods 1780 nasopharyngeal extracts from hospitalized children with acute respiratory infection in a women and children’s hospital in Chengdu from January 2010 to February 2014 were collected. The nested PCR method was used to screen the specimens with HRV-5’NCR primer , To know the total detection rate of the virus. The positive samples were further detected by HRV-VP4 / VP2 primers and the variation was analyzed by MEGA 6.06 software. Results Among 1 780 samples of nasopharyngeal aspirates, HRV was positive in 365 cases (20.5%, 365/1 780), of which 244 were positive for VP4 / VP2 gene. The results of sequence analysis showed that 132 cases were type A and 30 cases were Type B and type C in 82 cases. The detection rates of different genotypes fluctuated on the month and age, and type A and type C were epidemic characteristics. The homology analysis showed that the genotypes within and between genotypes Nucleotide homology is quite different. Conclusions HRV is one of the most common pathogens of acute respiratory infection in Chengdu. HRV-A and C are the major epidemics, with high variability among genotypes.