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为确定细胞类型和分化阶段是否能影响感染结果,本文研究牛痘病毒IHD-W株的穿入、脱壳、核酸杂交、蛋白质合成等,以探讨牛痘病毒在造血细胞系中的复制。用0.5～10PFU/细胞的病毒感染白血病K562细胞株、淋巴细胞NB104细胞株和早幼粒白血病HL60细胞株,然后测定细胞增殖与病毒感染性。在感染1、5、10PFU/细胞病毒的K562和NB104细胞株中,病毒效价增加,细胞溶解。将感染的K562株连续传代后,形成持续感染状态,但试图增殖感染的NB104株却失败了。而HL60株与对照细胞生长速度一样,当感染的HL60细胞
In order to determine whether the cell type and stage of differentiation affect the outcome of infection, we studied the penetration, shelling, nucleic acid hybridization and protein synthesis of vaccinia virus IHD-W in order to explore the replication of vaccinia virus in hematopoietic cell lines. The leukemia K562 cell line, the lymphocyte NB104 cell line and the promyelocytic leukemia HL60 cell line were infected with 0.5 to 10 PFU / cell of virus, and the cell proliferation and the virus infectivity were determined. In K562 and NB104 cell lines infected with 1, 5, 10 PFU / cell virus, the virus titer is increased and the cells lysis. The infected K562 strain was passaged continuously to form a persistent infection, but the NB104 strain that had been tried to multiply the infection failed. While the HL60 strain grew at the same rate as the control cells when infected with HL60 cells