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为了解各种因素对细胞热休克反应的影响,本文用Western印迹、蛋白水解酶活性电泳(renatured electrophoresis)、密度测定等方法分析了营养、血清、细胞密度等对C_6细胞合成热休克蛋白68(HSP68)量和时间的作用。结果表明,在一定血清浓度范围内(≤30％),培养基含的血清浓度越高,细胞启动HSP68合成的时间越长;营养条件的差异影响HSP68合成的量和时间;不同生长阶段的细胞热休克反应有差异,生长到稳定期的细胞是研究细胞应激反应的较合适材料。
In order to understand the effects of various factors on the heat shock response of cells, Western blotting, protein electrophoresis, density determination and other methods were used to analyze the effects of nutrition, serum, and cell density on the synthesis of heat shock protein 68 in C 6 cells ( HSP68) The amount and time effect. The results showed that within a certain serum concentration range (≤30%), the higher serum concentration in the culture medium, the longer the cells start HSP68 synthesis; the difference in nutritional conditions affects the amount and time of HSP68 synthesis; cells in different growth stages There are differences in the heat shock response. Cells that grow to a stable phase are suitable materials for studying cell stress responses.