东京医科齿科大学第三齿科保存教研室,从78年开始饲养无菌动物。由于无菌动物实验在饲养上弗事弗钱,对于大动物的应用是困难的。无菌动物一般免疫功能低下,形态变化也与普通动物完全一样,没有影响形态的缺点。但是却能够完全避免细菌的影响来进行实验,所以在牙髓疗法的范围,对于物理化学刺激引起的牙髓、牙周组织变化能够进行单纯的观察,从而获得可贵的实验材料。因此,该教研室开始用无菌大鼠。Kakeh-ashi等人1965年在美国口腔科杂志发表了同样试验。也就是将无菌大鼠和普通大鼠的右上第一磨牙的牙尖用19号金刚砂钻磨平,使牙髓暴露,不进行暂封,让其与口腔相通。术后4日,1、2、4周而且到6周杀死动物加以病 Department of Dental Dentistry, Tokyo Medical and Dental University Preservation of germ-free animals since 1978. Since aseptic animal experiments feed Freunds, it is difficult for large animal applications. Aseptic animals generally immune dysfunction, morphological changes are exactly the same with ordinary animals, there is no impact on the shortcomings of morphology. But can completely avoid the effects of bacteria to carry out experiments, so in the context of pulp therapy, for physical and chemical stimulation of pulp, periodontal tissue changes can be simply observed, so as to obtain valuable experimental materials. Therefore, the department began to use sterile rats. Kakeh-ashi et al. Published the same experiment in the American Journal of Stomatology in 1965. That is, aseptic rats and rats in the upper right first molar tooth cusp with No. 19 diamond grinding flat, so that the pulp exposed, no temporary closure, so that it communicates with the mouth. Animals were sacrificed on days 4, 1, 2, 4, and 6 weeks after surgery