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鞣酸可以捕获处于胞吐状态的内分泌颗粒,使之成为电镜下可观察到的高电子密度物质,从而精确定位胞吐部位和数量,本实验利用鞣酸固定法对应激状态大鼠脑下垂体前叶各种腺细胞的分泌状态做了超微结构观察,发现胞吐主要发生在催乳素细胞和促肾上腺皮质激素细胞上,而生长激素细胞、促性腺激素细胞和促甲状腺激素细胞少见。从每例样本中随机选取12个催乳素细胞和12个促肾上腺皮质激素细胞,分别统计细胞表面胞吐数目(以表示),催乳素细胞为1.16±0.16,促肾上腺皮质激素细胞为1.21±0.24,两组之间无显著差异(P>0.05),本实验从形态学上证实了大鼠胞下垂体前叶催乳素细胞和促肾上腺皮质激素细胞对应激反应的作用。
Tannin can capture endocrine granules in exocytosis, making it a high electron density material observable under electron microscopy to accurately locate the exocytosis site and the number of the use of tannin immobilization of stress state rat pituitary gland The secretion of various glandular cells in the anterior leaflet did ultrastructural observation and found that exocytosis mainly occurs on prolactin cells and adrenocorticotropic hormone cells, whereas growth hormone cells, gonadotropin cells and thyroid-stimulating hormone cells are rare. 12 prolactin cells and 12 adrenocorticotropic hormone cells were randomly selected from each sample, and the number of cell surface exocytosis was counted (expressed as), the number of prolactin cells was 1.16 ± 0.16, the level of adrenocorticotropic hormone-stimulated cells Was 1.21 ± 0.24, no significant difference between the two groups (P> 0.05), the experimental morphologically confirmed pro-pituitary prolactin and adrenocorticotropic hormone cells on the stress The role of reaction.