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目的建立广升麻药材快速分析方法。方法建立广升麻药材一致性模型与聚类分析模型,运用最小偏二乘法(PLS)建立广升麻中蜕皮甾酮含量与其近红外光谱之间的校正模型,并对未知样品进行含量预测。结果利用一致性模型与聚类分析模型能够准确的区分广升麻药材与其混用品,定量校正模型决定系数R2为92.34,内部交叉验证均方差(RMSECV)为0.94,外部验证均方差(RMSEP)为0.33。结论该方法准确、快速,为广升麻药材的快速分析方法的建立提供了科学依据。
OBJECTIVE To establish a rapid method for the analysis of Radix Astragali. Methods Consistency model and clustering analysis model of Radix copepagos were established. The calibration model of ecdysterone content in Radix Ophiopogonis was established by PLS method, and the prediction model of unknown samples was established. Results The concordance model and cluster analysis model could accurately distinguish Radix Astragali and its mixture, the coefficient of determination R2 of the quantitative calibration model was 92.34, RMSECV was 0.94, the root mean square error of external validation (RMSEP) was 0.33. Conclusion The method is accurate and rapid, which provides a scientific basis for the establishment of a rapid analytical method of Radix Cinnabar.