目的探讨氟康唑作用于白念珠菌双组分信号传导途径SSK1突变株SSK21后药物敏感性的变化。方法采用微量液体稀释法和固醇测定法测定野生株(CAF2-1)和突变株(SSK21)的最小抑菌浓度(MIC);并应用RT-PCR观察氟康唑作用前后,SSK1的表达变化。结果氟康唑对CAF2-1的MIC为16μg/mL,对SSK21为0.032μg/mL。加入氟康唑后,CAF2-1的SSK1表达明显增加,60min时达到最多。结论 SSK21对氟康唑高度敏感,SSK1基因及其相关的重要基因与药物敏感性的关系值得进一步研究,从而为新的抗真菌药物和治疗途径的研发提供参考。 Objective To investigate the effect of fluconazole on the susceptibility of SSK1 mutant SSK21 to Candida albicans, a two-component signal transduction pathway. Methods The minimum inhibitory concentration (MIC) of wild-type strain (CAF2-1) and mutant strain (SSK21) was determined by micro-fluid dilution method and sterol determination. The expression of SSK1 was detected by RT-PCR before and after treatment with fluconazole . Results The MIC of fluconazole to CAF2-1 was 16μg / mL and that to SSK21 was 0.032μg / mL. After adding fluconazole, the expression of SSK1 in CAF2-1 increased significantly and reached the maximum at 60min. Conclusion SSK21 is highly sensitive to fluconazole. The relationship between SSK1 gene and its related genes and drug sensitivity is worth further study, which will provide a reference for the development of new antifungal agents and therapeutic approaches.