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以大岩桐（ＳｉｎｎｉｇｉａｓｐｅｃｉｏｓａＢｅｎｔｈ．ｅｔＨｏｏｋ）叶片为试材，接种于ＭＳ附加不同激素组合的培养基上，成功地经愈伤组织亚球状体至不定芽途径形成大量再生植株并移栽成活。在各种细胞分裂素中，６－苄基嘌呤（ＢＡ）的效果优于激动素（ＫＴ）。生长素萘乙酸（ＮＡＡ）与ＢＡ及ＫＴ配合使用时愈伤组织诱导、球状体分化及不定芽增殖均有明显的增效作用。其最适宜的培养基组成为：ＭＳ＋ＢＡ２．０ｍｇ／Ｌ＋ＮＡＡ０．２ｍｇ／Ｌ在这个培养基上，愈伤组织诱导率为９４％，球状体分化率为１８８％，不定芽在口周内增殖５倍。最适宜的生极培养基为：ＭＳ＋ＮＡＡ０．５ｍｇ／Ｌ，生根率在９０％以上，试管苗移栽成活率在８０％以上。
The leaves of Sinnigiaspeciosa Benth.etHook were inoculated on MS medium supplemented with different hormones, and a large number of regenerated plants were successfully formed from the sub-spheroids to adventitious buds of callus and transplanted. Among various cytokinins, 6-benzylpurine (BA) is superior to kinetin (KT). The combination of NAA with BA and KT could induce callus induction, spheroid differentiation and adventitious bud proliferation. The most suitable medium consisted of MS + BA2.0mg / L + NAA0.2mg / L. On this medium, callus induction rate was94%, spheroid differentiation rate was188%, and adventitious buds multiplied by5times . The most suitable medium is: MS + NAA0.5mg / L, the rooting rate is more than 90%, the survival rate of test-tube seedling transplanting is more than 80%.