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采用MTT和SRB法研究了双核铂(Ⅱ)配合物[{Pt(H2O)2I}-μOOC-COO-{Pt(H2O)2I}](BPI)对人体癌细胞的增殖抑制作用,又通过流式细胞法、Giemsa染色法、等离子体质谱法研究了它的抗癌机制.实验结果表明:在10μmol.L-1浓度下,该配合物对HL-60,BGC-823,Bel-7402,KB,Hela,HCT-8,MCF-7和EJ 8种肿瘤细胞都表现出有较高的活性,对HL-60、BGC-823和Bel-7402 3种肿瘤细胞的抑制率都在70%以上,它能阻止HL-60细胞G2+M→G1期的进行;对HL-60细胞的凋亡诱导作用不明显;在相同浓度情况下其与HL-60细胞的DNA键合量大于顺铂.
The inhibition of the proliferation of human cancer cells by the dual-nuclear platinum (Ⅱ) complex [{Pt (H2O) 2I} -μOOC-COO- {Pt (H2O) 2I}] (BPI) was studied by MTT and SRB methods. Gc-823, Bel-7402, KB-1, Bcl-2 and Bcl-2 in the presence of 10μmol·L-1 by the method of Giemsa staining and plasma mass spectrometry Hela, HCT-8, MCF-7 and EJ all showed high activity. The inhibitory rates of the three kinds of tumor cells in HL-60, BGC-823 and Bel- It can prevent the G2 + M → G1 phase of HL-60 cells from proceeding; the apoptosis induction effect on HL-60 cells is not obvious; the amount of DNA binding to HL-60 cells is greater than that of cisplatin at the same concentration.