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观察表没食子儿茶素没食子酸酯(EGCG)对肾结石的治疗作用及对肾脏的保护作用,并对其可能机制进行探讨。采用乙二醇联合氯化铵灌胃的方法建立大鼠草酸钙肾结石模型,并以EGCG对其进行干预。采集大鼠血液标本,检测大鼠血肌酐、尿素氮以及血钙等生化指标;收集大鼠尿液标本,观察并比较大鼠24 h尿量、尿草酸以及尿钙水平;取大鼠肾脏标本制成组织切片,观察大鼠草酸钙肾结石的病理变化。采用实时定量PCR及Western blot对肾组织中骨桥蛋白(OPN)的表达水平进行检测。结果发现,与正常组大鼠相比,结石组大鼠血肌酐、尿素氮、尿钙、尿草酸水平以及肾组织内OPN表达水平均显著升高(P<0.05),而24 h尿量则显著降低(P<0.05);与肾结石组大鼠相比,在EGCG处理后,大鼠血肌酐、尿素氮、尿钙以及尿草酸水平显著降低(P<0.05),同时24 h尿量显著升高(P<0.05),呈浓度依赖性。与对照组相比,肾结石组大鼠肾组织草酸钙结石病理改变明显,而在EGCG处理后,大鼠肾脏病理改变明显改善,呈浓度依赖性。此外,EGCG处理后大鼠肾脏OPN表达水平显著降低,也呈浓度依赖性。结果可推知EGCG能够抑制大鼠肾脏内草酸钙肾结石的形成,并对肾功能有明显的保护作用。
To observe the therapeutic effect of epigallocatechin-3-gallate (EGCG) on kidney stones and its protective effect on the kidney, and to explore its possible mechanism. The model of calcium oxalate nephrolithiasis was established by gavage with ethylene glycol and ammonium chloride, and the model was induced by EGCG. Blood samples of rats were collected to detect serum creatinine, urea nitrogen and blood calcium and other biochemical indicators; urine samples were collected to observe and compare 24 h urine, urine oxalic acid and urinary calcium levels; rat kidney specimens Tissue sections were made to observe the pathological changes of calcium oxalate kidney stones in rats. The expression of osteopontin (OPN) in renal tissues was detected by real-time PCR and Western blot. The results showed that serum creatinine, urea nitrogen, urinary calcium and urinary oxalate levels and OPN expression in renal tissue were significantly increased (P <0.05), while those in 24 h urinary volume (P <0.05). Compared with the nephrolithiasis group, the levels of serum creatinine, urea nitrogen, urinary calcium and urinary oxalate were significantly decreased (P <0.05) (P <0.05), in a concentration-dependent manner. Compared with the control group, the pathological changes of kidney tissue calcium oxalate stones were obvious in the kidney stones group, while the pathological changes of the kidney in the rats after EGCG treatment significantly improved in a concentration-dependent manner. In addition, the expression of OPN in the kidneys of EGCG-treated rats was significantly decreased and also in a concentration-dependent manner. EGCG can be deduced from the results can inhibit the formation of kidney kidneys calcium oxalate stones, and renal function was significantly protective effect.