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目的了解重组的日本血吸虫中国大陆株脂肪酸结合蛋白(rSj-FABPc)的特性及预测其抗原表位。方法以PCR方法从Sj-cDNA文库中扩增Sj-FABPc基因片段，并亚克隆于载体pGEM-T，对其核苷酸序列进行测定。以GOLDKEY软件和Swiss-Model数据库分析Sj-FABPc的核苷酸序列和其编码的蛋白质特性，预测重组抗原的表位。结果核酸序列分析证明，克隆基因确为Sj-FABPc，并由此推导出Sj-FABPc理论氨基酸顺序，以该序列的分析表明，重组Sj-FABPc抗原具有3个抗原表位区和三个可能的脂肪酸结合表位。结论 Sj-FABPc抗原表位可能与脂肪酸结合表位的主要片段为同一肽段。“,”im To analyse the characteristic and antigen epitope of recombinant antigen Sj-FABPc, Methods The cloned gene of FABP was amplified by PCR from a Schistosoma japonicum adult worm cDNA library and subcloned into plasmid pGEM-T. The sequence was analysed by GOLDKEY DNA and Protein Analytical Program and Swiss-Model Protein Modelling server in Internet. Results The Tesult indicated that the cloned gene was Sj-FABPc. Three possible antigenic epitopes and three sites of binding fatty acid,of recombinant antigen of Sj-FABPc ,were predicted by computer. Conclusions The antigen epitopes of Sj-FABPc and the main sites of binding fatty acid may share same peptide.