论文部分内容阅读
以二氧化钛和硒化镉复合膜修饰的玻碳电极(TiO2/CdSe/GCE)作为工作电极,建立了一种测定甘氨酸的电化学发光(ECL)新方法.在强碱性溶液中,TiO2/CdSe/GCE在鲁米诺溶液中会产生明显的电化学发光信号,而甘氨酸对该体系的电化学发光信号有较强的增敏作用.考察了TiO2/CdSe复合物的比例、缓冲溶液的pH、发光试剂的浓度等条件对ECL强度的影响.最佳实验条件下,甘氨酸浓度在2.5×10-7 ～1.0×10-10 mol/L范围内与相对ECL强度呈现良好的线性关系,检测限(S/N=3)为3.3×10-1 mol/L,方法用于乳酸饮料中甘氨酸的测定,结果令人满意.“,”The Titanium Dioxide/CdSe quantum dots (TiO2/CdSe) compound was used to modify glassy carbon electrodes.The electrochemical luminescence of this modified electrode was studied.The results showed that the TiO2/CdSe modified electrode demonstrated an obvious ECL signal of luminol systems in alkaline media,and glycine can enhance electrochemical luminescence (ECL) signal of the luminol system.Based on this,a novel electrochemical luminescence method for the determination of glycine was developed.Several experiment factors,such as solid-liquid ratio of TiO2/CdSe compound,pH of buffer solution,and the concentration of luminol were selected.Under optimized conditions,the ECL signal intensity depended linearly on the concentration of glycine in 2.5 × 10-7-1.0× 10-10mol/L.The detection limit (S/N =3) was 3.3 × 10-11 mol/L.The proposed method was successfully applied to determine glycine in milk beverage.